Detection of porcine circovirus types 1 and 2 in serum and tissue samples of pigs with and without postweaning multisystemic wasting syndrome.

نویسندگان

  • Maria Calsamiglia
  • Joaquim Segalés
  • Josefina Quintana
  • Carles Rosell
  • Mariano Domingo
چکیده

Presence of porcine circovirus type 1 (PCV1) and PCV2 was studied in sera and superficial inguinal lymph nodes from postweaning multisystemic wasting syndrome (PMWS)-affected and non-PMWS-affected pigs by using in situ hybridization and PCR. PCV1 and PCV2 were found in less than 3% and more than 50% of the samples, respectively. The most sensitive technique and site was PCR in superficial inguinal lymph nodes, but in situ hybridization correlated better with presence of characteristic lesions.

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Quantitative, competitive PCR analysis of porcine circovirus DNA in serum from pigs with postweaning multisystemic wasting syndrome.

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عنوان ژورنال:
  • Journal of clinical microbiology

دوره 40 5  شماره 

صفحات  -

تاریخ انتشار 2002